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ナカシマ カズヒサ
Nakashima Kazuhisa
中島 和久 所属 鶴見大学 歯学部 歯学科 薬理学 職種 准教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Transgenic mice expressing a ligand-inducible cre recombinase in osteloblasts and odontoblasts - A new tool to examine physiology and disease of postnatal bone and tooth |
| 掲載誌名 | 正式名：AMERICAN JOURNAL OF PATHOLOGY ISSNコード：00029440 |
| 出版社 | AMER SOC INVESTIGATIVE PATHOLOGY, INC |
| 巻・号・頁 | 165(6),pp.1875-1882 |
| 著者・共著者 | JE Kim,K Nakashima,B de Crombrugghe |
| 発行年月 | 2004/12 |
| 概要 | The skeleton supports body structures in vertebrates and helps maintain calcium homeostasis throughout life. Disruption of genes involved in mammalian bone formation has often led to embryonic lethality, hence preventing study of these genes' role in adult animals. To develop a usable tool for such study, we generated transgenic micein which a 2.3-kb mouse Col1a1 proximal promoter, which is active in all osteoblasts, drives a transgene coding for a polypeptide consisting of Cre recombinase fused to a mutated ligand-binding domain of the estrogen receptor. In this Col1a1-CreERT2 mouse line, expression patterns of the transgene and of the resulting Cre-mediated DNA recombination are analyzed by crossing with ROSA26 reporter mice and by measurement of beta-galactosidase activity and X-gal staining. Exposure to 4-hydroxytamoxifen induced Cre-mediated recombination in osteoblasts in virtually all bones and in odontoblasts in teeth of both embryos and postnatal mice. The generation of these transgenic mice provides a new and important tool with which to study the function of specific genes in bone and tooth physiology and diseases in intact animals after birth. |