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コバヤシ サエコ
Kobayashi Saeko
小林 冴子 所属 鶴見大学 歯学部 歯学科 小児歯科学 職種 助教 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | DPP and DSP are Necessary for Maintaining TGF-beta 1 Activity in Dentin |
| 掲載誌名 | 正式名：JOURNAL OF DENTAL RESEARCH ISSNコード：00220345 |
| 出版社 | SAGE PUBLICATIONS INC |
| 巻・号・頁 | 93(7),pp.671-677 |
| 著者・共著者 | Y. Yamakoshi,S. Kinoshita,L. Izuhara,T. Karakida,M. Fukae,S. Oida |
| 発行年月 | 2014/07 |
| 概要 | Porcine dentin sialophosphoprotein (DSPP) is the most abundant non-collagenous protein in dentin. It is processed by proteases into 3 independent proteins: dentin sialoprotein (DSP), dentin glycoprotein (DGP), and dentin phosphoprotein (DPP). We fractionated DPP and DSP along with TGF-beta activity by ion exchange (IE) chromatography from developing pig molars and measured their alkaline phosphatase (ALP)-stimulating activity in human periodontal (HPDL) cells with or without TGF-beta receptor inhibitor. We then purified TGF-beta-unbound or -bound DPP and DSP by reverse-phase high-performance liquid chromatography (RP-HPLC) using the ALP-HPDL system. The TGF-beta isoform bound to DPP and DSP was identified as being TGF-beta 1 by both ELISA and LC-MS/MS analysis. We incubated carrier-free human recombinant TGF-beta 1 (CF-hTGF-beta 1) with TGF-beta-unbound DPP or DSP and characterized the binding on IE-HPLC using the ALP-HPDL system. When only CF-hTGF-beta 1 was incubated, approximately 3.6% of the ALP-stimulating activity remained. DPP and DSP rescued the loss of TGF-beta 1 activity. Approximately 19% and 10% of the ALP stimulating activities were retained by the binding of TGF-beta |
| DOI | 10.1177/0022034514534690 |