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マツモト ナオユキ
Matsumoto Naoyuki
松本 直行 所属 鶴見大学 歯学部 歯学科 病理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Detection of EGFR gene mutation by mutation-oriented LAMP method |
| 掲載誌名 | 正式名：Anticancer Research ISSNコード：17917530 |
| 出版社 | International Institute of Anticancer Research |
| 巻・号・頁 | 38(4),pp.2093-2099 |
| 著者・共著者 | Naoyuki Matsumoto,Akira Kumasaka,Tomohiro Ando,Kazuo Komiyama |
| 発行年月 | 2018/04 |
| 概要 | Background/Aim: Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for non-small cell lung cancer. EGFR gene mutations at codons 746-753 promote constitutive EGFR activation and result in worst prognosis. However, these mutations augment the therapeutic effect of EGFR-tyrosine kinase inhibitor. Therefore, the detection of EGFR gene mutations is important for determining treatment planning. The aim of the study was to establish a method to detect EGFR gene mutations at codons 746-753. Materials and Methods: EGFR gene mutation at codons 746-753 in six cancer cell lines were investigated. A loop-mediated isothermal amplification (LAMP)-based procedure was developed, that employed peptide nucleic acid to suppress amplification of the wild-type allele. Results: This mutation-oriented LAMP can amplify the DNA fragment of the EGFR gene with codons 746-753 mutations within 30 min. Moreover, boiled cells can work as template resources. Conclusion: Mutation oriented-LAMP assay for EGFR gene mutation is sensitive on extracted DNA. This procedure would be capable of detecting EGFR gene mutation in sputum, pleural effusion, broncho-alveolar lavage fluid or trans-bronc |
| DOI | 10.21873/anticanres.12449 |
| PMID | 29599327 |