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ハヤカワ トオル
Hayakawa Toru
早川 徹 所属 鶴見大学・鶴見大学短期大学部 学長・副学長 学長・副学長 副学長 職種 副学長 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Fibronectin immobilization using water-soluble carbodiimide on poly-L-lactic acid for enhancing initial fibroblast attachment. |
| 掲載誌名 | 正式名：Journal of biomaterials applications 略 称：J Biomater Appl ISSNコード：08853282/08853282 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 21(1),pp.33-47 |
| 著者・共著者 | Nagai Megumi, Hayakawa Tohru, Makimura Masaharu, Yoshinari Masao |
| 発行年月 | 2006/07 |
| 概要 | The aim of this study is to evaluate the influence of fibronectin immobilization on poly-L-lactic acid (PLA) films on the initial attachment of human gingival fibroblasts. Carboxylic acid groups are chemically introduced on the PLA films' surface by surface hydrolysis with 0.5 M NaOH. The contact angle of PLA surface with respect to double-distilled water decreases significantly after NaOH hydrolysis. X-ray photoelectron spectroscopy (XPS) also reveals significantly higher intensities of C(C=O)/C(C-O) after NaOH hydrolysis. Fibronectin is immobilized onto the hydrolyzed PLA surface through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLA surface and the amino groups of fibronectin using water-soluble carbodiimide. XPS analysis shows that the fibronectin-immobilized PLA surface is enriched with nitrogen atoms. The immobilization of fibronectin significantly enhances the number of initially attached human gingival fibroblasts on the PLA surface. |
| DOI | 10.1177/0885328206055316 |
| PMID | 16443618 |