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マツモト ナオユキ
Matsumoto Naoyuki
松本 直行 所属 鶴見大学 歯学部 歯学科 病理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | TGF-beta Signaling in Gingival Fibroblast-Epithelial Interaction |
| 掲載誌名 | 正式名：JOURNAL OF DENTAL RESEARCH ISSNコード：00220345 |
| 出版社 | SAGE PUBLICATIONS INC |
| 巻・号・頁 | 89(11),pp.1315-1321 |
| 著者・共著者 | M. Ohshima,Y. Yamaguchi,N. Matsumoto,P. Micke,Y. Takenouchi,T. Nishida,M. Kato,K. Komiyama,Y. Abiko,K. Ito,K. Otsuka,K. Kappert |
| 発行年月 | 2010/11 |
| 概要 | The underlying mechanism and the therapeutic regimen for the transition of reversible gingivitis to irreversible periodontitis are unclear. Since transforming growth factor (TGF)-beta has been implicated in differentially regulated gene expression in gingival fibroblasts, we hypothesized that TGF-beta signaling is activated in periodontitis-affected gingiva, along with enhanced collagen degradation, that is reversed by TGF-beta inhibition. A novel three-dimensional (3D) gel-culture system consisting of primary human gingival fibroblasts (GF) and gingival epithelial (GE) cells in collagen gels was applied. GF populations from patients with severe periodontitis degraded collagen gels, which was reduced by TGF-beta-receptor kinase inhibition. Up-regulation of TGF-beta-responsive genes was evident in GF/GE cocultures. Furthermore, the TGF-beta downstream transducer Smad3C was highly phosphorylated in periodontitis-affected gingiva and 3D cultures. These results imply that TGF-beta signaling is involved in fibroblast-epithelial cell interaction in periodontitis, and suggest that the 3D culture system is a useful in vitro model for therapeutic drug screening for periodontitis. |
| DOI | 10.1177/0022034510378423 |