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ナカヤマ リョウコ
Nakayama Ryoko
中山 亮子 所属 鶴見大学 歯学部 歯学科 病理学 職種 助教 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | A Competitive-ELISA for the Serodiagnosis of Anguillicolosis in Japanese Eel, Anguilla japonica. |
| 掲載誌名 | 正式名：Fish Pathology ISSNコード：0388788X |
| 出版社 | The Japanese Society of Fish Pathology |
| 巻・号・頁 | 34(1),pp.25-31 |
| 著者・共著者 | Inui, Takaya,Ushikoshi, Ryoko,Nogami, Sadao,Hirose, Hitomi |
| 発行年月 | 1999/03 |
| 概要 | Anguillicolosis is a parasitic disease of eel caused by infection with swimbladder nematode, Anguillicola crassus. This study aims to establish a serodiagnosis method for anguillicolosis. A competitive ELISA with an anti-Onchocerca volvulus mouse monoclonal antibody (No. 107) was applied to detect the specific antibodies against A. crassus in eel sera, using various capture antigens for ELISA. Infected eel sera showed a higher competitive reaction to the cuticle antigen of A. crassus than non-infected eel sera. The positive detection rate of infected eel sera and the false positive rate of non-infected eel sera were calculated as 95% and 0%, respectively. When an indirect ELISA with polyclonal rabbit anti-eel IgM antibodies was performed, the positive detection rate and the false positive rate were 80% and 20%, respectively. The competitive ELISA technique provided a more sensitive and specific serodiagnostic tool than the indirect ELISA for detecting A. crassus infection in eels. |
| DOI | 10.3147/jsfp.34.25 |
| PermalinkURL | http://joi.jlc.jst.go.jp/JST.Journalarchive/jsfp1966/34.25?from=CrossRef |