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サイトウ マリ
Saito Mari
齊藤 まり 所属 鶴見大学 歯学部 歯学科 分子生化学 職種 助教 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Differentiation potential of osteoblast from cultured C2C12 cells on zirconia disk |
| 掲載誌名 | 正式名：Dental Materials Journal ISSNコード：02874547 |
| 出版社 | The Japanese Society for Dental Materials and Devices |
| 巻・号・頁 | 33(2),pp.275-283 |
| 著者・共著者 | Saito, M.,Karakida, T.,Yamamoto, R.,Nagano, T.,Yamakoshi, Y.,Hayakawa, T.,Oida, S.,Gomi, K. |
| 発行年月 | 2014 |
| 概要 | We examined the adaptability of zirconia as a fixture for implants. A mouse myoblast cell line (C2C12) was seeded on Ce-TZP and titanium disks, and on poly-L-lysine-coated glass slides. Proliferation potency was determined by cell counting and mineral induction by BMP2 was studied. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and alizarin red (AIRS) staining. ALP activity and calcium concentrations were colorimetrically measured. The number of cells on all materials was approximately equal. ALP and AIRS staining showed densely-stained images, demonstrating the induction of C2C12 cells to express the osteoblastic phenotype. RT-PCR showed that mRNA expressions of type I collagen, osteocalcin, osterix and ALP were up-regulated. With regard to ALP activity and calcium concentration of C2C12 cells, no significant differences were observed between Ce-TZP and titanium disks. We conclude that Ce-TZP has the biological activity comparable to titanium and has the utility as fixture of dental implants. |
| DOI | 10.4012/dmj.2013-321 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84897058369&partnerID=MN8TOARS |