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サイトウ マリ
Saito Mari
齊藤 まり 所属 鶴見大学 歯学部 歯学科 分子生化学 職種 助教 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Combined Effect of Midazolam and Bone Morphogenetic Protein-2 for Differentiation Induction from C2C12 Myoblast Cells to Osteoblasts. |
| 掲載誌名 | 正式名：Pharmaceutics |
| 出版社 | {MDPI} {AG} |
| 巻・号・頁 | 12(3),pp.218-218 |
| 著者・共著者 | Yukihiko Hidaka,Risako Chiba-Ohkuma,Takeo Karakida,Kazuo Onuma,Ryuji Yamamoto,Keiko Fujii-Abe,Mari M Saito,Yasuo Yamakoshi,Hiroshi Kawahara |
| 発行年月 | 2020/03 |
| 概要 | In drug repositioning research, a new concept in drug discovery and new therapeutic opportunities have been identified for existing drugs. Midazolam (MDZ) is an anesthetic inducer used for general anesthesia. Here, we demonstrate the combined effects of bone morphogenetic protein-2 (BMP-2) and MDZ on osteogenic differentiation. An immortalized mouse myoblast cell line (C2C12 cell) was cultured in the combination of BMP-2 and MDZ (BMP-2+MDZ). The differentiation and signal transduction of C2C12 cells into osteoblasts were investigated at biological, immunohistochemical, and genetic cell levels. Mineralized nodules formed in C2C12 cells were characterized atthe crystal engineering level. BMP-2+MDZ treatment decreased the myotube cell formation of C2C12 cells, and enhanced alkaline phosphatase activity and expression levels of osteoblastic differentiation marker genes. The precipitated nodules consisted of randomly oriented hydroxyapatite nanorods and nanoparticles. BMP-2+MDZ treatment reduced the immunostaining for both α1 and γ2 subunits antigens on the gamma-aminobutyric acid type A (GABAA) receptor in C2C12 cells, but enhanced that for BMP signal transducers. Our investigation |
| DOI | 10.3390/pharmaceutics12030218 |
| PMID | 32131534 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-85081229689&partnerID=MN8TOARS |