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サイトウ マリ
Saito Mari
齊藤 まり 所属 鶴見大学 歯学部 歯学科 分子生化学 職種 助教 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Potential for Drug Repositioning of Midazolam for Dentin Regeneration. |
| 掲載誌名 | 正式名：International journal of molecular sciences |
| 巻・号・頁 | 20(3) |
| 著者・共著者 | Takeo Karakida,Kazuo Onuma,Mari M Saito,Ryuji Yamamoto,Toshie Chiba,Risako Chiba,Yukihiko Hidaka,Keiko Fujii-Abe,Hiroshi Kawahara,Yasuo Yamakoshi |
| 発行年月 | 2019/02 |
| 概要 | Drug repositioning promises the advantages of reducing costs and expediting approvalschedules. An induction of the anesthetic and sedative drug; midazolam (MDZ), regulatesinhibitory neurotransmitters in the vertebrate nervous system. In this study we show the potentialfor drug repositioning of MDZ for dentin regeneration. A porcine dental pulp-derived cell line(PPU-7) that we established was cultured in MDZ-only, the combination of MDZ with bonemorphogenetic protein 2, and the combination of MDZ with transforming growth factor-beta 1. Thedifferentiation of PPU-7 into odontoblasts was investigated at the cell biological and genetic level.Mineralized nodules formed in PPU-7 were characterized at the protein and crystal engineeringlevels. The MDZ-only treatment enhanced the alkaline phosphatase activity and mRNA levels ofodontoblast differentiation marker genes, and precipitated nodule formation containing a dentinspecificprotein (dentin phosphoprotein). The nodules consisted of randomly orientedhydroxyapatite nanorods and nanoparticles. The morphology, orientation, and chemicalcomposition of the hydroxyapatite crystals were similar to those of hydroxyapatite that hadtransformed from |
| DOI | 10.3390/ijms20030670 |
| PMID | 30720745 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-85061157950&partnerID=MN8TOARS |