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サイトウ マリ
Saito Mari
齊藤 まり 所属 鶴見大学 歯学部 歯学科 分子生化学 職種 助教 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Porcine keratin 75 in developing enamel. |
| 掲載誌名 | 正式名：Journal of oral biosciences ISSNコード：13490079 |
| 巻・号・頁 | 61(3),pp.163-172 |
| 著者・共著者 | Risako Chiba,Miu Okubo,Ryuji Yamamoto,Mari M Saito,Saeko Kobayashi,Elia Beniash,Yasuo Yamakoshi |
| 発行年月 | 2019/09 |
| 概要 | OBJECTIVE: To provide in vivo biochemical evidence for the isolation, identification, and characterization of porcine keratin 75 (K75) in developing enamel. METHODS: Immunolocalization of K75 was observed in mandibles from mice at postnatal days 5 and 11. K75 gene expression was analyzed by quantitative reverse transcription-polymerase chain reaction using enamel organ epithelium (EOE) of incisors from pigs at 5 months of age. Enamel protein was extracted and isolated from both immature and mature enamel of second molars from 5-month-old pigs, and the K75 antibody-positive fraction was analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). In vitro protease digestion of K75-antibody-positive fraction was carried out using porcine kallikrein 4 (pKLK4) or recombinant human enamelysin (rhMMP20) and their degradation patterns were characterized by both SDS-PAGE and western blotting. RESULTS: Specific immunostaining for K75 was restricted to the layers of stratum intermedium and the enamel side of ameloblasts in mice at postnatal day 5, and to the papillary layer at postnatal day 11. Porcine K75 was expressed throughout enamel formation, but its transcript levels were signifi |
| DOI | 10.1016/j.job.2019.06.002 |
| PMID | 31252053 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-85068365162&partnerID=MN8TOARS |