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ナカシマ カズヒサ
Nakashima Kazuhisa
中島 和久 所属 鶴見大学 歯学部 歯学科 薬理学 職種 准教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Essential roles of G9a in cell proliferation and differentiation during tooth development |
| 掲載誌名 | 正式名：EXPERIMENTAL CELL RESEARCH ISSNコード：00144827 |
| 出版社 | ELSEVIER INC |
| 巻・号・頁 | 357(2),pp.202-210 |
| 著者・共著者 | Taichi Kamiunten,Hisashi Ideno,Akemi Shimada,Yoshinori Arai,Tatsuo Terashima,Yasuhiro Tomooka,Yoshiki Nakamura,Kazuhisa Nakashima,Hiroshi Kimura,Yoichi Shinkai,Makoto Tachibana,Akira Nifuji |
| 発行年月 | 2017/08 |
| 概要 | Teeth develop through interactions between epithelial and mesenchymal tissues mediated by a signaling network comprised of growth factors and transcription factors.However, little is known about how epigenetic modifiers affect signaling pathways and thereby regulate tooth formation. We previously reported that the histone 3 lysine 9 (H3K9) methyltransferase (MTase) G9a is specifically enriched in the tooth mesenchyme during mouse development. In this study, we investigated thefunctions of G9a in tooth development using G9a conditional knockout (KO) mice. We used Sox9-Cre mice to delete G9a in the tooth mesenchyme because Sox9 is highly expressed in the mesenchyme derived from the cranial neural crest. Immunohistochemical analyses revealed that G9a expression was significantly decreased in the mesenchyme of Sox9-Cre;G9afl/fl (G9a cKO) mice compared with that in Sox9-Cre;G9a fl/+(control) mice. Protein levels of the G9a substrate H3K9me2 were also decreased in the tooth mesenchyme. G9a cK0 mice showed smaller tooth germ after embryonic day (E) 16.5 and E17.5, but not at E15.5. The developing cusp tips, which were visible in control mice, were absent in G9a cKO mice at E17.5. At 3 w |
| DOI | 10.1016/j.yexcr.2017.05.016 |
| PMID | 28527696 |