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ナカシマ カズヒサ
Nakashima Kazuhisa
中島 和久 所属 鶴見大学 歯学部 歯学科 薬理学 職種 准教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Efficient expansion of mouse primary tenocytes using a novel collagen gel culture method |
| 掲載誌名 | 正式名：HISTOCHEMISTRY AND CELL BIOLOGY ISSNコード：09486143 |
| 出版社 | SPRINGER |
| 巻・号・頁 | 142(2),pp.205-215 |
| 著者・共著者 | Akemi Shimada,Satoshi Wada,Kouji Inoue,Hisashi Ideno,Taichi Kamiunten,Koichiro Komatsu,Akira Kudo,Yoshiki Nakamura,Tetsuji Sato,Kazuhisa Nakashima,Akira Nifuji |
| 発行年月 | 2014/08 |
| 概要 | Development of regenerative therapies for damaged tendons remains a great challenge, largely because of lack of information regarding the mechanisms responsible for differentiation of tenocytes. Mouse tenocytes have not been fully characterized owing to the absence of efficient and reproducible methods for their in vitro expansion without losing phenotypic features. The objective of the study was to establish an improved and reliable method for stable primary culture of mouse tenocytes by using collagen gel. Achilles and tail tendon tissues were harvested and embedded in collagen gel. After 10 days of continuous culture, the gel was digested and cells were passaged on tissue culture-treated plastic dishes. Mouse tenocytes cultured in collagen gel exhibited significantly shorter doubling time and higher numbers of proliferation when maintained on the plastic dishes compared with those cultured without using gel. Transmission electron microscopic analyses showed that cultured tenocytes retained some morphological features of tenocytes in tendon tissues, such as cell-cell junctional complex formation, well-developed rough endoplasmic reticulum, and mitochondria in their cytoplasm. mRN |
| DOI | 10.1007/s00418-014-1191-4 |