|
ナカシマ カズヒサ
Nakashima Kazuhisa
中島 和久 所属 鶴見大学 歯学部 歯学科 薬理学 職種 准教授 |
|
| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Expression analysis of LacZ gene placed in the locus of cnot7 exhibits its activity in osteoblasts in vivo and in mineralized nodules in vitro |
| 掲載誌名 | 正式名：JOURNAL OF CELLULAR BIOCHEMISTRY ISSNコード：07302312 |
| 出版社 | WILEY-LISS |
| 巻・号・頁 | 99(2),pp.538-544 |
| 著者・共著者 | Kaoru Washio-Oikawa,Takahisa Nakamura,Michihiko Usui,Mitsuhiro Yoneda,Youichi Ezura,Isao Ishikawa,Kazuhisa Nakashima,Tadashi Yamamoto,Masaki Noda |
| 発行年月 | 2006/10 |
| 概要 | CCR4-NOT complex 7 (Cnot7) was identified as a regulator of gene expression in yeast and evolutionally conserved in mammals. Cnot7 deficient male mice exhibit abnormality in spermatogenesis. As these mice contained construct to express LacZ, we followed the expression patterning in these animals. LacZ was expressed in osteoblasts located in the primary spongiosa in adult mice. Cellular analysis indicated that LacZis expressed in osteoblasts but not in osteoclasts. In the mineralized nodules formed in the culture of bone marrow cells obtained from Cnot7+/- mice, LacZ expression was mainly observed in the cells forming mineralized nodules but not in un-mineralized area scattered around the periphery of the nodules. LacZ blue positive cells were gradually depositing minerals along its time course of the in vitro mineralization assay. Cnot7expression was enhanced by the treatment with BMP. These data suggest that Cnot7 is expressed in osteoblasts and is associated with mineralization. |
| DOI | 10.1002/jcb.20854 |
| PMID | 16639713 |