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ナカシマ カズヒサ
Nakashima Kazuhisa
中島 和久 所属 鶴見大学 歯学部 歯学科 薬理学 職種 准教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Bone morphogenetic protein-2 enhances osterix gene expression in chondrocytes |
| 掲載誌名 | 正式名：JOURNAL OF CELLULAR BIOCHEMISTRY ISSNコード：07302312 |
| 出版社 | WILEY-LISS |
| 巻・号・頁 | 88(6),pp.1077-1083 |
| 著者・共著者 | K Yagi,K Tsuji,A Nifuji,K Shinomiya,K Nakashima,B deCrombrugghe,M Noda |
| 発行年月 | 2003/04 |
| 概要 | Osterix is a recently identified zinc-finger-containing transcription factor, which is required for skeleto-genesis as no bone formation was observed in osterix-deficient mice. Osterix was first cloned as a gene whose expression was enhanced by BMP in C2C12 cells. As BMP induces ectopic bone formation in vivo via a pathway reminiscent to endochondral bone formation, BMP may also regulate osterix gene expression in chondrocytes. However, no information was available regarding the BMP actions on osterix gene expression in chondrocytes. We therefore examined the effects of BMP-2 on osterix gene expression in chondrocytes in culture. RT-PCR analysis indicated that osterix m RNA was expressed in the primary cultures of chondrocytes derived from mouse rib cartilage. The treatment with BMP-2 enhanced the levels of osterix transcripts within 24 h and the enhancement was still observed at 48 h based on RT-PCR analysis. This BMP effect was specific to this cytokine, as TGF-beta did not alter osterix gene expression. BMP effects on the osterix mRNA levels were also confirmed by Northern blot analysis. The enhancing effect of BMP on osterix gene expression was observed in a dose-dependent mann |
| DOI | 10.1002/jcb.10467 |