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ナカシマ カズヒサ
Nakashima Kazuhisa
中島 和久 所属 鶴見大学 歯学部 歯学科 薬理学 職種 准教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Osteoclast-Specific Dicer Gene Deficiency Suppresses Osteoclastic Bone Resorption |
| 掲載誌名 | 正式名：JOURNAL OF CELLULAR BIOCHEMISTRY ISSNコード：07302312 |
| 出版社 | WILEY-LISS |
| 巻・号・頁 | 109(5),pp.866-875 |
| 著者・共著者 | Fumitaka Mizoguchi,Yayoi Izu,Tadayoshi Hayata,Hiroaki Hemmi,Kazuhisa Nakashima,Takashi Nakamura,Shigeaki Kato,Nobuyuki Miyasaka,Yoichi Ezura,Masaki Noda |
| 発行年月 | 2010/04 |
| 概要 | Osteoclasts are unique cells that resort) bone, and are involved in not only bone remodeling but also pathological bone loss such as osteoporosis and rheumatoid arthritis. The regulation of osteoclasts is based on a number of molecules but full details of these molecules have not yet been understood. MicroRNAs are producedby Dicer cleavage an emerging regulatory system for cell and tissue function. Here, we examine the effects of Dicer deficiency in osteoclasts on osteoclastic activity and bone mass in vivo. We specifically knocked out Dicer in osteoclasts by crossing Dicer flox mice with cathepsin K-Cre knock-in mice. Dicer deficiency in osteoclasts decreased the number of osteoclasts (N.Oc/BS) and osteoclast surface (Oc.S/BS) in vivo. Intrinsically, Dicer deficiency in osteoclasts suppressed the levels of TRAP positive multinucleated cell development in culture and also reduced NFATc1 and TRAP gene expression. MicroRNA analysis indicated that expression of miR-155 was suppressed by RANKL treatment in Dicer deficient cells. Dicer deficiency in osteoclasts suppressed osteoblastic activity in vivo including mineral apposition rate (MAR) and bone formation rate (BFR) and also suppre |
| DOI | 10.1002/jcb.22228 |