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ナカシマ カズヒサ
Nakashima Kazuhisa
中島 和久 所属 鶴見大学 歯学部 歯学科 薬理学 職種 准教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Tumor necrosis factor-α enhances RANKL expression in gingival epithelial cells via protein kinase A signaling |
| 掲載誌名 | 正式名：Journal of Periodontal Research ISSNコード：16000765 |
| 出版社 | Blackwell Munksgaard |
| 巻・号・頁 | 49(4),pp.508-517 |
| 著者・共著者 | R. Fujihara,M. Usui,G. Yamamoto,K. Nishii,Y. Tsukamoto,Y. Okamatsu,T. Sato,Y. Asou,K. Nakashima,M. Yamamoto |
| 発行年月 | 2014 |
| 概要 | Objective and Background: Periodontitis is an inflammatory disorder of the supporting tissue of teeth, which is composed of gingival soft tissue, cementum covering the tooth root, alveolar bone and periodontal ligament. The receptor activator of nuclear factor kappa B ligand (RANKL) is known to be an essential factor for osteoclastogenesis. Recent clinical studies indicate that levels of RANKL in the gingival crevicular fluid are increased while levels of its decoy receptor, osteoprotegerin (OPG), are decreased in patients with periodontitis. Although the gingival sulcus is composed of gingival tissue, RANKL and OPG expression in gingival epithelial cells is not fully understood. The aim of this study is to investigate the expression of RANKL and OPG in gingival tissue and which factors regulate RANKL expression in gingival epithelial cells. Material and Methods: Reverse transcriptase polymerase chain reaction analysis, western blotting and immunohistochemistry were performed to confirm RANKL and OPG expression in gingival epithelial cells (GECs) and in gingival tissue. Immunostaining was also examined to confirm tumor necrosis factor (TNF)-α and TNF receptor type 1 (TNFR1) expres |
| DOI | 10.1111/jre.12131 |
| PMID | 24102429 |