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ニフジ アキラ
Nifuji Akira
二藤 彰 所属 鶴見大学 歯学部 歯学科 薬理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | H3K9MTase G9a is essential for the differentiation and growth of tenocytes in vitro |
| 掲載誌名 | 正式名：HISTOCHEMISTRY AND CELL BIOLOGY ISSNコード：09486143 |
| 出版社 | SPRINGER |
| 巻・号・頁 | 144(1),pp.13-20 |
| 著者・共著者 | Satoshi Wada,Hisashi Ideno,Akemi Shimada,Taichi Kamiunten,Yoshiki Nakamura,Kazuhisa Nakashima,Hiroshi Kimura,Yoichi Shinkai,Makoto Tachibana,Akira Nifuji |
| 発行年月 | 2015/07 |
| 概要 | Cell differentiation is controlled by specific transcriptionfactors. The functions and expression levels of these transcription factors are regulated by epigenetic modifications, such as histone modifications and cytosine methylation of the genome. In tendon tissue, tendon-specific transcription factors have been shown to play functional roles in the regulation of tenocyte differentiation. However, the effects of epigenetic modifications on gene expression and differentiation in tenocytes are unclear. In this study, we investigated the epigenetic regulation of tenocyte differentiation, focusing on the enzymes mediating histone 3 lysine 9 (H3K9) methylation. In primary mouse tenocytes, six H3K9 methyltransferase (H3K9MTase) genes, i.e., G9a, G9a-like protein (GLP), PR domain zinc finger protein 2 (PRDM2), SUV39H1, SUV39H2, and SETDB1/ESET were all expressed, with increased mRNA levels observed during tenocyte differentiation. In mouse embryos, G9a and Prdm2 mRNAs were expressed in tenocyte precursor cells, which were overlapped with or were adjacent to cells expressing a tenocyte-specific marker, tenomodulin. Using tenocytes isolated from G9a-flox/flox mice, we deleted G9a by infec |
| DOI | 10.1007/s00418-015-1318-2 |
| PMID | 25812847 |