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ニフジ アキラ
Nifuji Akira
二藤 彰 所属 鶴見大学 歯学部 歯学科 薬理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Spaciotemporal association and bone morphogenetic protein regulation of Sclerostin and Osterix expression during embryonic osteogenesis |
| 掲載誌名 | 正式名：ENDOCRINOLOGY ISSNコード：00137227 |
| 出版社 | ENDOCRINE SOC |
| 巻・号・頁 | 145(10),pp.4685-4692 |
| 著者・共著者 | Y Ohyama,A Nifuji,Y Maeda,T Amagasa,M Noda |
| 発行年月 | 2004/10 |
| 概要 | Sclerostin (SOST), a member of the cystine-knot superfamily, is essential for proper skeletogenesis because a loss-of-function mutation in the SOST gene results in sclerosteosis featured with massive bone growth in humans. To understand the function of SOST in developmental skeletal tissue formation, we examined SOST gene expression in embryonic osteogenesis in vitro and in vivo. During osteoblastic differentiation in primary calvarial cells, the levels of SOST expression were increased along with those of alkaline phosphatase activity and nodule formation. In situ hybridization study revealed that SOST mRNA expression was observed in the digits in embryonic 13-d limb buds, and SOST expression was observed in osteogenic front in embryonic 16.5-d postcoitus embryonic calvariae, and this expression persisted in the peripheral area of cranial bone in the later developmental stage (embryonic 18.5-d post coitum). These temporal and spacial expression patterns in vivo and in vitro were in parallel to those of osterix (Osx), which is a critical transcriptional factor for bone formation. Similar coexpression of SOST and Osx mRNA was observed when the primary osteoblastic calvarial cells we |
| DOI | 10.1210/en.2003-1492 |