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ニフジ アキラ
Nifuji Akira
二藤 彰 所属 鶴見大学 歯学部 歯学科 薬理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Establishment of tendon-derived cell lines exhibiting pluripotent mesenchymal stem cell-like property |
| 掲載誌名 | 正式名：EXPERIMENTAL CELL RESEARCH ISSNコード：00144827 |
| 出版社 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 287(2),pp.289-300 |
| 著者・共著者 | R Salingcarnboriboon,H Yoshitake,K Tsuji,M Obinata,T Amagasa,A Nifuji,MNoda |
| 発行年月 | 2003/07 |
| 概要 | Development of the musculoskeletal system requires coordinated formation of distinct types of tissues, including bone, cartilage, muscle, and tendon. Compared to muscle, cartilage, and bone, cellular and molecular bases of tendon development have not been well understood due to the lack of tendon cell lines. The purpose of this study was to establish and characterize tendon cell lines. Three clonal tendon cell lines (TT-E4, TT-G11, and TT-D6) were established using transgenic mice harboring a temperature-sensitive mutant of SV40 large T antigen. Proliferation of these cells was significantly enhanced by treatment with bFGF and TGF-beta but not BMP2. Tendon phenotype-related genes such as those encoding scleraxis, Six1, EphA4, COMP, and type I collagen were expressed in these tendon cell clones. In addition to tendon phenotype-related genes, expression of osteopontin and Cbfal was observed. These clonal cell lines formed hard fibrous connective tissue when implanted onto chorioallantoic membrane in ovo. Furthermore, these cells also formed tendon-like tissues when they were implanted into defects made in patella tendon in mice. As these tendon cell lines also produced fibrocartilagi |
| DOI | 10.1016/S0014-4827(03)00107-1 |