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ニフジ アキラ
Nifuji Akira
二藤 彰 所属 鶴見大学 歯学部 歯学科 薬理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Noggin expression in a mesodermal pluripotent cell line C1 and its regulation by BMP |
| 掲載誌名 | 正式名：Journal of Cellular Biochemistry ISSNコード：07302312 |
| 巻・号・頁 | 73(4),pp.437-444 |
| 著者・共著者 | Akira Nifuji,Odile Kellermann,Masaki Noda |
| 発行年月 | 1999/06 |
| 概要 | Osteoblasts and chondrocytes are derived from mesodermal stem cells and their differentiation is under the control of coordinated interaction among signaling molecules. Noggin is one of the signaling molecules which bind to and inactivate BMPs to induce neural tissues and dorsal mesoderm in Xenopus. However, its expression and regulation in mammalian cells has not been known. In this study, we investigated expression of noggin in murine pluripotent mesodermal cell line, C1. Noggin expression was very low in these C1 cells before they were induced to differentiate. When C1 cells were induced to differentiate into chondrocytes in aggregate cultures in the presence of dexamethasone(dex), noggin expression was significantly increased. In a sharp contrast, when the C1 cells were induced to differentiate into osteoblastic cells by the treatment with β glycerophosphate (βGP) and ascorbic acid (AA), noggin mRNA expression remained to be barely detectable. Noggin expression was also observed in the developing cartilage of vertebrae in 15.5 dpc mouse embryos. The noggin mRNA level in C1 cells in monolayer cultures was enhanced significantly by the treatment with BMP4/7 in a dose-dependent |
| DOI | 10.1002/(SICI)1097-4644(19990615)73:4<437::AID-JCB2>3.0.CO;2-B |
| PMID | 10733338 |