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イデノ ヒサシ
Ideno Hisashi
出野 尚 所属 鶴見大学 歯学部 歯学科 薬理学 職種 講師 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Alendronate promotes bone formation by inhibiting protein prenylation in osteoblasts in rat tooth replantation model |
| 掲載誌名 | 正式名：JOURNAL OF ENDOCRINOLOGY ISSNコード：00220795 |
| 出版社 | BIOSCIENTIFICA LTD |
| 巻・号・頁 | 219(2),pp.145-158 |
| 著者・共著者 | Koichiro Komatsu,Akemi Shimada,Tatsuya Shibata,Satoshi Wada,Hisashi Ideno,Kazuhisa Nakashima,Norio Amizuka,Masaki Noda,Akira Nifuji |
| 発行年月 | 2013/11 |
| 概要 | Bisphosphonates (BPs) are a major class of antiresorptive drug, and their molecular mechanisms of antiresorptive action have been extensively studied. Recent studies have suggested that BPs target bone-forming cells as well as bone-resorbing cells. We previously demonstrated that local application of a nitrogen-containing BP (N-BP), alendronate (ALN), for a short period of time increased bone tissue in a rat tooth replantation model. Here, we investigated cellular mechanisms of bone formation by ALN. Bone histomorphometry confirmed that bone formation was increased by local application of ALN. ALN increased proliferation of bone-forming cells residing on the bone surface, whereas it suppressed the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in vivo. Moreover, ALN treatment induced more alkaline phosphatase-positive and osteocalcin-positive cells on the bone surface than PBS treatment. In vitro studies revealed that pulse treatment with ALN promoted osteocalcin expression. To track the target cells of N-BPs, we applied fluorescence-labeled ALN (F-ALN) in vivo and in vitro. F-ALN was taken into bone-forming cells both in vivo and in vitro. This intracell |
| DOI | 10.1530/JOE-13-0040 |
| PMID | 24096963 |