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マツモト ナオユキ
Matsumoto Naoyuki
松本 直行 所属 鶴見大学 歯学部 歯学科 病理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Rapid and Specific Screening Assay for KRAS Oncogene Mutation by a Novel Gene Amplification Method |
| 掲載誌名 | 正式名：ANTICANCER RESEARCH ISSNコード：02507005 |
| 出版社 | INT INST ANTICANCER RESEARCH |
| 巻・号・頁 | 36(4),pp.1571-1579 |
| 著者・共著者 | Akira Kumasaka,Naoyuki Matsumoto,Shotaro Mukae,Taiichi Kitano,Hiroyasu Noguchi,Hidero Ohki,Kazuo Komiyama,Tomohiro Ando |
| 発行年月 | 2016/04 |
| 概要 | Background: Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for colorectal cancer. However, mutation of Kirsten rat sarcoma viral oncogene homolog (KRAS) gene at codons 12 and 13 attenuates the therapeutic effect of anti-EGFR therapies. Therefore, the detection of KRAS gene mutation is important for therapeutic decision-making. Materials and Methods: KRAS gene mutation at codons 12 (c.34G>T, c.35G>C, c.35G>A) and 13 (c.38G>A) in six cancer cell lines were investigated. A loop-mediated isothermal amplification-based procedure was developed that employed peptide nucleic acid to suppress amplification of the wild-type allele. Results: This mutation-oriented gene-amplification procedure can amplify the DNA fragment of the KRAS gene with codon 12 and codon 13 mutation within 30 min. Moreover, boiled cells can work as template resources. Conclusion: This newly developed procedure can be useful for patient stratification for anti-EGFR therapies. |