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マツモト ナオユキ
Matsumoto Naoyuki
松本 直行 所属 鶴見大学 歯学部 歯学科 病理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Multiple cleavage sites for polymeric immunoglobulin receptor |
| 掲載誌名 | 正式名：IMMUNOLOGY ISSNコード：00192805 |
| 出版社 | BLACKWELL PUBLISHING LTD |
| 巻・号・頁 | 112(4),pp.583-589 |
| 著者・共著者 | M Asano,N Takenouchi-Ohkubo,N Matsumoto,Y Ogura,H Nomura,H Suguro,Moro, I |
| 発行年月 | 2004/08 |
| 概要 | Human polymeric immunoglobulin receptor (pIgR) was expressed in baby hamster kidney (BHK) cells using a recombinant vaccinia virus transfection system. Cleavage of pIgR on the cell surface was partially inhibited by the proteinase inhibitor, leupeptin. We addressed the question whether some particular regions of pIgR could affect the efficient cleavage of this molecule, with the following results: (1) a mutant lacking the entire cytoplasmic region resulted in release of secretory component (SC) into the culture supernatant much faster than wild-type; (2) a pIgR mutant lacking the entire extracellular domain 6, the region containing the susceptible cleavage sites, could be cleaved and released as a mutant SC. The transport kinetics of this mutant between endoplasmic reticulum (ER) and Golgi or Golgi and the cell surface was equivalent to wild-type pIgR. Our results indicate that although the main cleavage site is in domain 6, at least one other cleavage site may exist. |
| DOI | 10.1046/j.1365-2567.2004.01914.x |