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マツモト ナオユキ
Matsumoto Naoyuki
松本 直行 所属 鶴見大学 歯学部 歯学科 病理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Release of non-glycosylated polymeric immunoglobulin receptor protein |
| 掲載誌名 | 正式名：SCANDINAVIAN JOURNAL OF IMMUNOLOGY ISSNコード：03009475 |
| 出版社 | BLACKWELL PUBLISHING LTD |
| 巻・号・頁 | 58(4),pp.471-476 |
| 著者・共著者 | N Matsumoto,M Asano,Y Ogura,N Takenouchi-Ohkubo,H Chihaya,W Chung-Hsing,K Ishikawa,L Zhu,Moro, I |
| 発行年月 | 2003/10 |
| 概要 | Using a recombinant vaccinia virus containing the T7 RNA polymerase, we have established a system for the transient expression of human polymeric immunoglobulin receptor (pIgR) in baby hamster kidney cells, a baby hamster-derived fibroblastic cell line. This transfection system resulted in the successful expression of pIgR in these cells, and Western blot analysis showed that human pIgR was expressed as two different molecular weight forms of 92 and 107 kDa. Treatment with endoglycosidase H showed that the difference between these two forms was due to the glycosylation status of the protein. In order to examine the functional role of glycosylation, we treated the transfected cells with tunicamycin, which prevents a core glycosylation step in the endoplasmic reticulum. Non-glycosylated pIgR was released into the culture medium of the transfected cells, albeit with extremely low efficiency. Taking these results together, we conclude that the glycosylation of pIgR may play a positive role in the efficient transport or release of free pIgR. |
| DOI | 10.1046/j.1365-3083.2003.01325.x |