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マツモト ナオユキ
Matsumoto Naoyuki
松本 直行 所属 鶴見大学 歯学部 歯学科 病理学 職種 教授 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Cloning and expression of the turtle (Trachemys scripta) immunoglobulin joining (J)-chain cDNA |
| 掲載誌名 | 正式名：Immunogenetics ISSNコード：00937711 |
| 出版社 | Springer Science and Business Media LLC |
| 巻・号・頁 | 54(7),pp.513-519 |
| 著者・共著者 | Arihiro Iwata,Takashi Iwase,Yoshitaka Ogura,Tomihisa Takahashi,Naoyuki Matsumoto,Toshiyuki Yoshida,Nobuhiro Kamei,Kunihiko Kobayashi,Jiri Mestecky,Itaru Moro |
| 発行年月 | 2002 |
| 概要 | The J-chain protein is a Mr 15,000 polypeptide associated with polymeric IgA and IgM. The complete cDNA sequences of human, mouse, cow, brushtail possum, chicken and frog J chains have been previously reported, but nothing is known about the cDNA and amino acid sequences of reptilian J chain. Here, we determined a turtle J-chain cDNA sequence by RT-PCR and RACE, and examined J-chain mRNA and protein expression by Northern blotting and immunohistochemistry. This turtle J-chain cDNA was 1,934 bp and had an open reading frame of 477 nucleotides, encoding 159 amino acids. The mature J-chain protein is composed of 137 amino acids, Mr ∼15,000. The deduced amino acid sequence of the turtle J chain was highly homologous to that of human (60%), mouse (61%), cow (60%), rabbit (60%), chicken (69%), brushtail possum (65%), Rana catesbeiana (47%) and Xenopus laevis (58%). Eight cysteine residues were located at the same positions as in these other species, with the exception of X. laevis. PROSITE database analysis indicated the presence of two N-glycosylation sites in turtle, one of which was novel. Northern blot analysis revealed that turtle J-chain mRNA was expressed in lung, stomach, splee |
| DOI | 10.1007/s00251-002-0492-2 |
| PMID | 12389099 |
| PermalinkURL | http://link.springer.com/content/pdf/10.1007/s00251-002-0492-2.pdf |
| researchmap用URL | http://link.springer.com/article/10.1007/s00251-002-0492-2/fulltext.html |