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ハヤカワ トオル
Hayakawa Toru
早川 徹 所属 鶴見大学・鶴見大学短期大学部 学長・副学長 学長・副学長 副学長 職種 副学長 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide. |
| 掲載誌名 | 正式名：Dental materials journal 略 称：Dent Mater J ISSNコード：02874547/02874547 |
| 掲載区分 | 国内 |
| 巻・号・頁 | 27(5),pp.744-750 |
| 著者・共著者 | Yamamichi Nobuyuki, Pugdee Kamolparn, Chang Wei-Jen, Lee Sheng-Yang, Yoshinari Masao, Hayakawa Tohru, Abiko Yoshimitsu |
| 発行年月 | 2008/09 |
| 概要 | Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy. |
| DOI | 10.4012/dmj.27.744 |
| PMID | 18972793 |