|
ハマダ ヨシキ
Hamada Yoshiki
濱田 良樹 所属 鶴見大学 歯学部 歯学科 口腔顎顔面外科学 職種 教授 |
|
| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Treatment of salivary gland hypofunction by transplantation with dental pulp cells |
| 掲載誌名 | 正式名：ARCHIVES OF ORAL BIOLOGY ISSNコード：00039969 |
| 出版社 | PERGAMON-ELSEVIER SCIENCE LTD |
| 巻・号・頁 | 58(8),pp.935-942 |
| 著者・共著者 | Yuka Yamamura,Hiroyuki Yamada,Takashi Sakurai,Fumio Ide,Hiroko Inoue,Takashi Muramatsu,Kenji Mishima,Yoshiki Hamada,Ichiro Saito |
| 発行年月 | 2013/08 |
| 概要 | Objective: This study aimed to establish a mouse model in which dental pulp cells (DPCs) could be used as a cell source for the treatment of salivary gland hypofunction.Design: DPCs were isolated from green fluorescent protein (GFP)-expressing mice and were differentiated into dental pulp endothelial cells (DPECs). DPEC behaviour was studied in vitro and in vivo to investigate their capacity to participate in neovascularisation. For in vivo assessment, a combination of DPECs and Matrigel was subcutaneously injected into nude mice. Two weeks after injection, Matrigel plugs were analysed for CD31 and GFP. Furthermore, both submandibular glands of the irradiated mice were injected with DPECs. Eight weeks after irradiation, the effect of DPECs on saliva secretion was evaluated by measuring amounts of saliva secretion.Results: DPECs showed typical endothelial morphology, including a cobblestone appearance. RT-PCR analysis of DPECs showed positive expression of CD31, foetal liver kinase-1, vascular-endothelial-cadherin, vascular endothelial growth factor-A and von Willebrand factor. DPECs reorganised into tube-like structures on Matrigel after 24 h in vitro. Positive merged staining fo |
| DOI | 10.1016/j.archoralbio.2013.02.015 |
| PMID | 23570866 |