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コバヤシ サエコ
Kobayashi Saeko
小林 冴子 所属 鶴見大学 歯学部 歯学科 小児歯科学 職種 助教 |
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| 論文種別 | 【査読あり】 研究論文（学術雑誌） |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | TGF-beta 1 autocrine signalling and enamel matrix components |
| 掲載誌名 | 正式名：SCIENTIFIC REPORTS ISSNコード：20452322 |
| 出版社 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 6 |
| 著者・共著者 | Saeko Kobayashi-Kinoshita,Yasuo Yamakoshi,Kazuo Onuma,Ryuji Yamamoto,Yoshinobu Asada |
| 発行年月 | 2016/09 |
| 概要 | Transforming growth factor-beta 1 (TGF-beta 1) is present in porcine enamel extracts and is critical for proper mineralization of tooth enamel. Here, we show that the mRNA of latent TGF-beta 1 is expressed throughout amelogenesis. Latent TGF-beta 1 is activated by matrix metalloproteinase 20 (MMP20), coinciding with amelogenin processing by the same proteinase. Activated TGF-beta 1 binds to the major amelogenin cleavage products, particularly the neutral-soluble P103 amelogenin, to maintain its activity. The P103 amelogenin-TGF-beta 1 complex binds to TGFBR1 to induce TGF-beta 1 signalling. The P103 amelogenin-TGF-beta 1 complex is slowly cleaved by kallikrein 4 (KLK4), which is secreted into the transition-and maturation-stage enamel matrix, thereby reducing TGF-beta 1 activity. To exert the multiple biological functions of TGF-beta 1 for amelogenesis, we propose that TGF-beta 1 is activated or inactivated by MMP20 or KLK4 and that the amelogenin cleavage product is necessary for the in-solution mobility of TGF-beta 1, which is necessary for binding to its receptor on ameloblasts and retention of its activity. |
| DOI | 10.1038/srep33644 |